Generator

Part:BBa_K1773021:Design

Designed by: Sarunas Tumas   Group: iGEM15_Vilnius-Lithuania   (2015-08-26)


Cas3 protein with pLuxcI right promoter and weak RBS.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2718
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1439
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2402
    Illegal AgeI site found at 2775
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

no


Source

I-F type CRISPR-Cas system was cloned from Aggregatibacter actinomycetemcomitans D7-S strand.

References